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KMID : 0613820110210010022
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Journal of Life Science
2011 Volume.21 No. 1 p.22 ~ p.30
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Effects of Apolipoprotein A-I on Apoptosis and Cytokine Production in Human Neutrophils
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Kang Hyung-Gon
Choi Jae-Hyung
Huh Jae-Taeck
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Abstract
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Apolipoprotein A-I (apoA-I) has anti-inflammatory and anti-oxidative properties. This study was designed to investigate whether apoA-I affects apoptosis and cytokine production of human blood neutrophils in an in vitro culture system. Spontaneous apoptosis of neutrophils was significantly delayed by apoA-I. In addition, high density lipoprotein containing apoA-I also delayed apoptosis of neutrophils. Apoptosis of neutrophils was inhibited by anti-scavenger receptor type B-I antibodies. The amounts of interleukin-8, interferon (IFN)-inducible protein 10 (IP-10), and tumor necrosis factor-α (TNF-α) in the supernatants of cultured neutrophils treated with apoA-I were significantly increased. Combined treatment of neutrophils with IFN-γ and apoA-I produced higher amounts of IP-10 and TNF-α than did treatment with IFN-γ or apoA-I alone. The present study reveals that apoA-I activates neutrophils to produce cytokines and delays spontaneous apoptosis of neutrophils. These findings suggest that apoA-I, although a well-known negative acute-phase protein, has a pro-inflammatory effect in neutrophils.
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KEYWORD
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Neutrophils, apoptosis, apolipoprotein A-I, tumor necrosis factor-α (TNF-α)
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